In case the above did not fix your issue:
Please do the following in Google Chrome to ensure that the permissions are properly enabled: You must allow your browser to access your microphone and speakers before being able to accept or make a call in Talkdesk. Advanced Microphone Permissions Troubleshooting.There are several configuration steps that we recommend following on your browser or machine to prevent this error message from appearing: If you are getting the error 'Unable to access microphone, please check system settings.' while using Callbar, this is because certain permissions were not given on the browser, or your headset is not properly plugged in or recognized on your machine.